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prolong gold antifade solution containing dapi  (Thermo Fisher)


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    Structured Review

    Thermo Fisher prolong gold antifade solution containing dapi
    Prolong Gold Antifade Solution Containing Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prolong+gold+antifade+solution+containing+dapi/pm39806101-446-9-10
    Average 90 stars, based on 1 article reviews
    prolong gold antifade solution containing dapi - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microscopy:

    Article Title: Specialized pericyte subtypes in the pulmonary capillaries.
    Article Snippet: The day before the analysis, 25 μL of sheep anti-rat IgG Dynabeads (11035; Invitrogen, Carlsbad, CA) were incubated overnight with 10 μg of CD45 IgG. .. Samples were then washed again and placed on microscopy slides with Prolong Gold Antifade Solution containing DAPI (Life Technologies Corporation). .. The tissue was then minced and digested using a Miltenyi GentleMACS dissociator (Miltenyi Biotec, Germany).

    In Vitro:

    Article Title: HYDROGEL-BASED DELIVERY OF IL-10 IMPROVES TREATMENT OF BLEOMYCIN-INDUCED LUNG FIBROSIS IN MICE
    Article Snippet: EZ-Link Maleimide-PEG 2 -Biotin was purchased from Thermo-Fischer Scientific (21901BID), reconstituted based on manufacturer protocol, and combined with HH at manufacturer protocol suggested concentration. .. The following antibodies and reagents were used for paraffin embedded mouse lung tissues, as well as in vitro formalin fixed cell culture: F-Actin-488 conjugated (1:250 IF, A12379; Life Technologies Corporation); Collagen-1 (1:100 IF, 1:750 WB, NB600–408; Novus Biotechnologies); α-SMA (1:300 IHC, AB5694; Abcam); pSmad3 (1:400 IHC, 118825; Abcam); Hyaluronic Acid Binding Protein (1:100 IHC, 385911; Millipore); EZLink Maleimide-PEG 2 -Biotin (21901BID; Thermo Scientific); Anti-Human IL-10 (1:200 IHC, ab9969;Abcam); STAT3 (1:500; Cell Signalling Technology, cat. #12640); Prolong Gold Antifade Solution containing DAPI (Life Technologies Corporation). .. Total cellular RNA was extracted from flash frozen healthy control and bleomycin mouse lung tissue using the RNeasy Mini Kit (Qiagen, Redwood City, CA) and converted to cDNA using the Fermentas Reverse Transcription Kit (Vilnius, Lithuania) following the manufacturer’s protocol.

    Cell Culture:

    Article Title: HYDROGEL-BASED DELIVERY OF IL-10 IMPROVES TREATMENT OF BLEOMYCIN-INDUCED LUNG FIBROSIS IN MICE
    Article Snippet: EZ-Link Maleimide-PEG 2 -Biotin was purchased from Thermo-Fischer Scientific (21901BID), reconstituted based on manufacturer protocol, and combined with HH at manufacturer protocol suggested concentration. .. The following antibodies and reagents were used for paraffin embedded mouse lung tissues, as well as in vitro formalin fixed cell culture: F-Actin-488 conjugated (1:250 IF, A12379; Life Technologies Corporation); Collagen-1 (1:100 IF, 1:750 WB, NB600–408; Novus Biotechnologies); α-SMA (1:300 IHC, AB5694; Abcam); pSmad3 (1:400 IHC, 118825; Abcam); Hyaluronic Acid Binding Protein (1:100 IHC, 385911; Millipore); EZLink Maleimide-PEG 2 -Biotin (21901BID; Thermo Scientific); Anti-Human IL-10 (1:200 IHC, ab9969;Abcam); STAT3 (1:500; Cell Signalling Technology, cat. #12640); Prolong Gold Antifade Solution containing DAPI (Life Technologies Corporation). .. Total cellular RNA was extracted from flash frozen healthy control and bleomycin mouse lung tissue using the RNeasy Mini Kit (Qiagen, Redwood City, CA) and converted to cDNA using the Fermentas Reverse Transcription Kit (Vilnius, Lithuania) following the manufacturer’s protocol.

    Western Blot:

    Article Title: HYDROGEL-BASED DELIVERY OF IL-10 IMPROVES TREATMENT OF BLEOMYCIN-INDUCED LUNG FIBROSIS IN MICE
    Article Snippet: EZ-Link Maleimide-PEG 2 -Biotin was purchased from Thermo-Fischer Scientific (21901BID), reconstituted based on manufacturer protocol, and combined with HH at manufacturer protocol suggested concentration. .. The following antibodies and reagents were used for paraffin embedded mouse lung tissues, as well as in vitro formalin fixed cell culture: F-Actin-488 conjugated (1:250 IF, A12379; Life Technologies Corporation); Collagen-1 (1:100 IF, 1:750 WB, NB600–408; Novus Biotechnologies); α-SMA (1:300 IHC, AB5694; Abcam); pSmad3 (1:400 IHC, 118825; Abcam); Hyaluronic Acid Binding Protein (1:100 IHC, 385911; Millipore); EZLink Maleimide-PEG 2 -Biotin (21901BID; Thermo Scientific); Anti-Human IL-10 (1:200 IHC, ab9969;Abcam); STAT3 (1:500; Cell Signalling Technology, cat. #12640); Prolong Gold Antifade Solution containing DAPI (Life Technologies Corporation). .. Total cellular RNA was extracted from flash frozen healthy control and bleomycin mouse lung tissue using the RNeasy Mini Kit (Qiagen, Redwood City, CA) and converted to cDNA using the Fermentas Reverse Transcription Kit (Vilnius, Lithuania) following the manufacturer’s protocol.

    Immunohistochemistry:

    Article Title: HYDROGEL-BASED DELIVERY OF IL-10 IMPROVES TREATMENT OF BLEOMYCIN-INDUCED LUNG FIBROSIS IN MICE
    Article Snippet: EZ-Link Maleimide-PEG 2 -Biotin was purchased from Thermo-Fischer Scientific (21901BID), reconstituted based on manufacturer protocol, and combined with HH at manufacturer protocol suggested concentration. .. The following antibodies and reagents were used for paraffin embedded mouse lung tissues, as well as in vitro formalin fixed cell culture: F-Actin-488 conjugated (1:250 IF, A12379; Life Technologies Corporation); Collagen-1 (1:100 IF, 1:750 WB, NB600–408; Novus Biotechnologies); α-SMA (1:300 IHC, AB5694; Abcam); pSmad3 (1:400 IHC, 118825; Abcam); Hyaluronic Acid Binding Protein (1:100 IHC, 385911; Millipore); EZLink Maleimide-PEG 2 -Biotin (21901BID; Thermo Scientific); Anti-Human IL-10 (1:200 IHC, ab9969;Abcam); STAT3 (1:500; Cell Signalling Technology, cat. #12640); Prolong Gold Antifade Solution containing DAPI (Life Technologies Corporation). .. Total cellular RNA was extracted from flash frozen healthy control and bleomycin mouse lung tissue using the RNeasy Mini Kit (Qiagen, Redwood City, CA) and converted to cDNA using the Fermentas Reverse Transcription Kit (Vilnius, Lithuania) following the manufacturer’s protocol.

    Binding Assay:

    Article Title: HYDROGEL-BASED DELIVERY OF IL-10 IMPROVES TREATMENT OF BLEOMYCIN-INDUCED LUNG FIBROSIS IN MICE
    Article Snippet: EZ-Link Maleimide-PEG 2 -Biotin was purchased from Thermo-Fischer Scientific (21901BID), reconstituted based on manufacturer protocol, and combined with HH at manufacturer protocol suggested concentration. .. The following antibodies and reagents were used for paraffin embedded mouse lung tissues, as well as in vitro formalin fixed cell culture: F-Actin-488 conjugated (1:250 IF, A12379; Life Technologies Corporation); Collagen-1 (1:100 IF, 1:750 WB, NB600–408; Novus Biotechnologies); α-SMA (1:300 IHC, AB5694; Abcam); pSmad3 (1:400 IHC, 118825; Abcam); Hyaluronic Acid Binding Protein (1:100 IHC, 385911; Millipore); EZLink Maleimide-PEG 2 -Biotin (21901BID; Thermo Scientific); Anti-Human IL-10 (1:200 IHC, ab9969;Abcam); STAT3 (1:500; Cell Signalling Technology, cat. #12640); Prolong Gold Antifade Solution containing DAPI (Life Technologies Corporation). .. Total cellular RNA was extracted from flash frozen healthy control and bleomycin mouse lung tissue using the RNeasy Mini Kit (Qiagen, Redwood City, CA) and converted to cDNA using the Fermentas Reverse Transcription Kit (Vilnius, Lithuania) following the manufacturer’s protocol.



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    HMGB1 and TLR-4 expressions in the lung tissue of injured control and MSC-treated swine. The swine were exposed to smoke inhalation and burn injury, then treated with saline (injury control), or autologous MSC treatment (MSC), and observation for up to 72 h PI. (A) Lung tissue samples were obtained during necropsy and proceeded for immunohistochemistry (IHC) staining, the primary antibodies of anti-HMGB1 (rabbit anti-HMGB1), and anti-TLR4 (mouse anti-TLR4), followed by visualization with a goat anti-rabbit IgG conjugated with Cy3 (red) and goat anti-mouse IgG conjugated with cy2 (green), respectively. DNA was stained with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI)</t> (blue). Scale bar = 50 μm. Note that the co-localized staining (yellow arrows) of HMGB1 and TLR4 was observed in the injury control but not in the MSC treatment group. (B) The mean immunofluorescence signal intensity in the IHC images was measured by ImageJ software and presented as mean± SEM. n=5 swine per experimental group was analyzed. Statistical analysis was performed using the Mann–Whitney U test, *p<0.05.
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    HMGB1 and TLR-4 expressions in the lung tissue of injured control and MSC-treated swine. The swine were exposed to smoke inhalation and burn injury, then treated with saline (injury control), or autologous MSC treatment (MSC), and observation for up to 72 h PI. (A) Lung tissue samples were obtained during necropsy and proceeded for immunohistochemistry (IHC) staining, the primary antibodies of anti-HMGB1 (rabbit anti-HMGB1), and anti-TLR4 (mouse anti-TLR4), followed by visualization with a goat anti-rabbit IgG conjugated with Cy3 (red) and goat anti-mouse IgG conjugated with cy2 (green), respectively. DNA was stained with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI)</t> (blue). Scale bar = 50 μm. Note that the co-localized staining (yellow arrows) of HMGB1 and TLR4 was observed in the injury control but not in the MSC treatment group. (B) The mean immunofluorescence signal intensity in the IHC images was measured by ImageJ software and presented as mean± SEM. n=5 swine per experimental group was analyzed. Statistical analysis was performed using the Mann–Whitney U test, *p<0.05.
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    HMGB1 and TLR-4 expressions in the lung tissue of injured control and MSC-treated swine. The swine were exposed to smoke inhalation and burn injury, then treated with saline (injury control), or autologous MSC treatment (MSC), and observation for up to 72 h PI. (A) Lung tissue samples were obtained during necropsy and proceeded for immunohistochemistry (IHC) staining, the primary antibodies of anti-HMGB1 (rabbit anti-HMGB1), and anti-TLR4 (mouse anti-TLR4), followed by visualization with a goat anti-rabbit IgG conjugated with Cy3 (red) and goat anti-mouse IgG conjugated with cy2 (green), respectively. DNA was stained with <t>4′,6-diamidino-2-phenylindole</t> <t>(DAPI)</t> (blue). Scale bar = 50 μm. Note that the co-localized staining (yellow arrows) of HMGB1 and TLR4 was observed in the injury control but not in the MSC treatment group. (B) The mean immunofluorescence signal intensity in the IHC images was measured by ImageJ software and presented as mean± SEM. n=5 swine per experimental group was analyzed. Statistical analysis was performed using the Mann–Whitney U test, *p<0.05.
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    Image Search Results


    HMGB1 and TLR-4 expressions in the lung tissue of injured control and MSC-treated swine. The swine were exposed to smoke inhalation and burn injury, then treated with saline (injury control), or autologous MSC treatment (MSC), and observation for up to 72 h PI. (A) Lung tissue samples were obtained during necropsy and proceeded for immunohistochemistry (IHC) staining, the primary antibodies of anti-HMGB1 (rabbit anti-HMGB1), and anti-TLR4 (mouse anti-TLR4), followed by visualization with a goat anti-rabbit IgG conjugated with Cy3 (red) and goat anti-mouse IgG conjugated with cy2 (green), respectively. DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar = 50 μm. Note that the co-localized staining (yellow arrows) of HMGB1 and TLR4 was observed in the injury control but not in the MSC treatment group. (B) The mean immunofluorescence signal intensity in the IHC images was measured by ImageJ software and presented as mean± SEM. n=5 swine per experimental group was analyzed. Statistical analysis was performed using the Mann–Whitney U test, *p<0.05.

    Journal: Frontiers in Immunology

    Article Title: Circulatory HMGB1 is an early predictive and prognostic biomarker of ARDS and mortality in a swine model of polytrauma

    doi: 10.3389/fimmu.2023.1227751

    Figure Lengend Snippet: HMGB1 and TLR-4 expressions in the lung tissue of injured control and MSC-treated swine. The swine were exposed to smoke inhalation and burn injury, then treated with saline (injury control), or autologous MSC treatment (MSC), and observation for up to 72 h PI. (A) Lung tissue samples were obtained during necropsy and proceeded for immunohistochemistry (IHC) staining, the primary antibodies of anti-HMGB1 (rabbit anti-HMGB1), and anti-TLR4 (mouse anti-TLR4), followed by visualization with a goat anti-rabbit IgG conjugated with Cy3 (red) and goat anti-mouse IgG conjugated with cy2 (green), respectively. DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar = 50 μm. Note that the co-localized staining (yellow arrows) of HMGB1 and TLR4 was observed in the injury control but not in the MSC treatment group. (B) The mean immunofluorescence signal intensity in the IHC images was measured by ImageJ software and presented as mean± SEM. n=5 swine per experimental group was analyzed. Statistical analysis was performed using the Mann–Whitney U test, *p<0.05.

    Article Snippet: After washing, sections were mounted with ProLong Gold Antifade solution containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Carlsbad, CA) for staining the nuclear DNA.

    Techniques: Immunohistochemistry, Staining, Immunofluorescence, Software, MANN-WHITNEY